CABRI Guidelines

LABORATORY PROCEDURES FOR MICROORGANISMS

Part 1: Introduction and general documents

Procedures

Appendixes

Part 2: Request and handling of deposits

Flow diagram

Procedures

Appendixes

Part 3: Maintaining deposits

Flow diagrams

Procedures

Appendixes

Part 4: Delivery of material to customers

Flow diagram

Procedures

Appendixes

Part 1: Introduction and general documents - Procedures -

Reference Number	Procedure Title
M/1999/1.00	Introduction & general documents
M/1999/1.01	Establishment and operation of culture collections
M/1999/1.02	International laws and regulations concerning the collection work
M/1999/1.03	European laws and regulations concerning the collection work
M/1998/1.04	National laws and regulations concerning the collection work
M/1998/1.05	Security, quality and transfer restriction codes
M/1998/1.06	Introduction to safety regulations
M/1998/1.07	Issue, control and updating of laboratory procedures
M/1998/1.08	Traceability of data

Part 1: Introduction and general documents - Appendixes -

Reference Number	Appendixes
M/1999/1.00 Appendix 1	WFCC Guidelines for the Establishment and Operation of Collections of Cultures of Microorganisms
M/1998/1.05 Appendix 1	Potentially pathogenic materials
M/1998/1.05 Appendix 2	Potentially hallucenogenic materials
M/1998/1.05 Appendix 3	Potential toxin producers
M/1998/1.05 Appendix 4	Transfer agreement
M/1998/1.06 Appendix 1	Safe handling of liquid nitrogen
M/1998/1.06 Appendix 2	First aid: Laboratory acquired infections
M/1998/1.06 Appendix 3	Good Laboratory Practice
M/1998/1.06 Appendix 4	Unpacking of cultures received for deposit

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Part 2: Guidelines for requesting and handling new deposits - Procedures -

Reference Number	Procedure Title
M/1998/2.00	Accession of Microorganisms
M/1998/2.01	Accession form
M/1998/2.02	Recording collection data
M/1998/2.03	Transfer of cultures
M/1998/2.04	Storage of original culture material
M/1998/2.05	(Preliminary) quality check
M/1998/2.06	Acknowledgement of receipt of a deposit
M/1998/2.07	Depositor check
M/1998/2.08	Release of cultures for dispatch
M/1998/2.09	Transfer of strain data to database

Part 2: Guidelines for requesting and handling new deposits - Appendixes -

Reference Number	Appendixes
M/1998/2.00 Appendix 2.1	Accession criteria BCCM
M/1998/2.00 Appendix 2.2	Accession criteria CBS
M/1998/2.00 Appendix 2.3	Accession criteria DSMZ
M/1998/2.00 Appendix 3.1	Standard letter: Request of strain
M/1998/2.00 Appendix 4.1	Standard letter: Rejection of strain
M/1998/2.01 Appendix 1	Accession form BCCM-LMG
M/1998/2.01 Appendix 2	Accession form BCCM-IHEM
M/1998/2.01 Appendix 3	Accession form BCCM-MUCL
M/1998/2.01 Appendix 4	Accession form CBS
M/1998/2.01 Appendix 5	Accession form DSMZ
M/1998/2.02 Appendix 1	Day Book, example DSMZ
M/1998/2.02 Appendix 2	Accession Book
M/1998/2.03 Appendix 1	Preparation and storage of media
M/1998/2.03 Appendix 2	Preparation of prereduced media
M/1998/2.05 Appendix 1	Selected tests for quality control
M/1998/2.05 Appendix 2	Procedure for preliminary quality check
M/1998/2.06 Appendix 1	Standard letter
M/1998/2.07 Appendix 1	Standard letter
M/1998/2.07 Appendix 2	Depositor's reply form

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Part 3: Guidelines for maintaining deposits - Flow diagrams -

Reference Number	Flow diagrams
M/1998/3.00 Appendix 1	Maintenance and preservation
M/1998/3.00 Appendix 2	Preservation methods
M/1998/3.00 Appendix 5.07.1	Ultra-low cryopreservation
M/1998/3.00 Appendix 5.08.1	Freeze-drying process
M/1998/3.00 Appendix 5.10.1	Centrifugal freeze-drying
M/1998/3.00 Appendix 5.11.1	Vacuum-drying
M/1998/3.00 Appendix 5.13.1	Freeze drying of fungi
M/1998/3.02 Appendix 1	Monitoring viability and stock numbers
M/1998/3.02 Appendix 2	Stock control, frozen cultures
M/1998/3.02 Appendix 3	Stock control, (freeze)-dried cultures

Part 3: Guidelines for maintaining deposits - Procedures -

Reference Number	Procedure Title
M/1998/3.00	Maintenance and Preservation of Microorganisms
M/1998/3.01	Storage of preserved microorganisms
M/1998/3.02	Stock control and periodic quality control

Part 3: Guidelines for maintaining deposits - Appendixes -

Reference Number	Appendixes
M/1998/3.00 Appendix 3	Protective suspension media for freezing or (freeze)-drying
M/1998/3.00 Appendix 4	Protocol form: Protective suspension media
M/1998/3.00 Appendix 5.00	Survey of detailed preservation methods
M/1998/3.00 Appendix 5.01	Preservation of bacteria by freezing and low temperature storage in glass capillary tubes
M/1998/3.00 Appendix 5.01.1	Protocol form: Preservation of bacteria in LN2
M/1998/3.00 Appendix 5.01.2	Protocol form: Quantity of frozen stock cultures
M/1998/3.00 Appendix 5.01.3	Protocol form: Position of cultures in LN2
M/1998/3.00 Appendix 5.02	Preservation of bacteria by freezing and low temperature storage in polypropylene straws
M/1998/3.00 Appendix 5.03	Preservation of sporoactinomyces by freezing and low temperature storage in PVC straws
M/1998/3.00 Appendix 5.04	Preservation of bacteria above liquid nitrogen in cryotubes
M/1998/3.00 Appendix 5.05	Preservation of bacteria by freezing and low temperature storage below -70°C on glass beads
M/1998/3.00 Appendix 5.05.1	Protocol Form: Preservation of bacteria by freezing and storage below -70°C on glass beads
M/1998/3.00 Appendix 5.06	Preservation of bacteria on ceramic beads below -70°C
M/1998/3.00 Appendix 5.07	Cryopreservation of fungi
M/1998/3.00 Appendix 5.07.2	Comments: Ultra-low cryopreservation
M/1998/3.00 Appendix 5.08	Preservation of bacteria by freeze-drying (1)
M/1998/3.00 Appendix 5.08.2	Protocol form: (Freeze)-drying of bacteria
M/1998/3.00 Appendix 5.09	Preservation of bacteria by freeze-drying (2)
M/1998/3.00 Appendix 5.10	Preservation of bacteria by centrifugal freeze-drying
M/1998/3.00 Appendix 5.11	Preservation of bacteria by vacuum-drying
M/1998/3.00 Appendix 5.11.2	Protocol form: Predried skim milk batches
M/1998/3.00 Appendix 5.12	Preservation of bacteria by liquid-drying (L-drying)
M/1998/3.00 Appendix 5.13	Preservation of fungi by freeze-drying
M/1998/3.00 Appendix 5.13.2	Comments: Freeze drying of fungi
M/1998/3.00 Appendix 5.14	Opening of ampoules and rehydration of (freeze)-dried cultures
M/1998/3.02 Appendix 4	Procedure for estimating survival rates: bacteria, yeasts
M/1998/3.02 Appendix 5	Procedures estimation of survival after preservation: fungi
M/1998/3.02 Appendix 6	Viability check book
M/1998/3.02 Appendix 7	Preservation book
M/1998/3.02 Appendix 8	Note for entering cultures into the stock/delivery datafile

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Part 4: Guidelines for delivering material to customers - Procedures -

Reference Number	Procedure Title
M/1998/4.00	Ordering and dispatch of cultures
M/1998/4.01	Procedure for dealing with customers complaints

Part 4: Guidelines for delivering material to customers - Appendixes -

Reference Number	Appendixes
M/1998/4.01 Appendix 1	Form: Complaints

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Guidelines prepared for CABRI by DSMZ, CBS and BCCM, 17 May 1998; updated August 1999
Page layout by CERDIC
Copyright CABRI, 1999

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