CABRI Guidelines

LABORATORY PROCEDURES FOR PLANT CELL LINES

Part 1: Introduction and general documents

Procedures

Appendixes

Part 2: Request and handling new deposits

Flow diagram

Procedures

Appendixes

Part 3: Maintaining deposits

Procedures

Appendixes

Part 4: Delivery of material to the customer

Procedures

Appendixes

Part 1: Introduction and general documents - Procedures -

Reference Nb.	Procedure Title
PC/1998/1/1	General Guidelines for Management and Organisation
PC/1998/1/2	General Principals and Guidelines
PC/1998/1/3	International Laws and Regulations Concerning the Collection Work
PC/1998/1/4	European Laws and Regulations Concerning the Collection Work
PC/1998/1/5	National Laws and Regulations Concerning the Collection Work
PC/1998/1/6	Security, Quality and Transfer Restriction Codes
PC/1998/1/7	Introduction to safety regulations
PC/1998/1/8	Issue, control and updating of laboratory procedures
PC/1998/1/9	Review of Laboratory Record Books and Protocol Forms

Part 1: Introduction and general documents - Appendixes -

Reference Nb.	Appendixes
PC/1999/1/2 Appendix 1	WFCC Guidelines for the Establishment and Operation of Collections of Cultures of Micro-organisms
PC/1998/1/7 Appendix 1	Safe Handling of Liquid Nitrogen
PC/1998/1/7 Appendix 2	First Aid for Laboratory Infections
PC/1998/1/7 Appendix 3	Good Microbiological Practice

Top of Page

Part 2: Guidelines for requesting and handling new deposits - Procedures -

Reference Nb.	Procedure Title
PC/1998/2/1	Guidelines for Requesting, Processing, Cataloguing, Maintenance and Delivery of Plant Cell Lines
PC/1998/2/1.1	Flow chart: Requesting, Processing, Maintenance and Delivery of Plant Cell
PC/1998/2/2	Request and receipt of plant cell lines
PC/1998/2/2.1	Definition of Accession criteria
PC/1998/2/2.2	Description of Accession Form
PC/1998/2/2.3	Day Book
PC/1998/2/2.4	Allocation of Accession Number
PC/1998/2/3	Preliminary Quality Control
PC/1998/2/3.1	Macroscopic and Microscopic Examination
PC/1998/2/3.2	Test for Fungal and Yeast Contaminants
PC/1998/2/3.3	Test for Bacterial Contaminants
PC/1998/2/3.4	Viability test: TTC
PC/1998/2/3.5	Viability test: FDA

Part 2: Guidelines for requesting and handling new deposits - Appendixes -

Reference Nb.	Appendixes
PC/1998/2/2 Appendix 1	Standard letter: Request of a plant cell line
PC/1998/2/2 Appendix 2	Standard letter: Acknowledgement of Accession Form
PC/1998/2/2 Appendix 3	Standard letter: Asking for alternative methods
PC/1998/2/2 Appendix 4	Standard letter: Acknowledgement receipt of accession form
PC/1998/2/2 Appendix 5	Standard letter: Acknowledgement of receipt cell line
PC/1998/2/2.1 Appendix 1	Accession criteria
PC/1998/2/2.2 Appendix 1	Accession Form
PC/1998/2/2.3 Appendix 1	Day Book, exemple
PC/1998/2/3 Appendix 1	Standard Letter: Cell line contaminated
PC/1998/2/3 Appendix 2	Standard Letter: Low Viability 1
PC/1998/2/3 Appendix 3	Standard Letter: Low Viability 2
PC/1998/2/3 Appendix 4	Standard Letter: no Viability
PC/1998/2/3.2 Appendix 1	Laboratory Procedure for Yeast and Fungi Test Agar
PC/1998/2/3.3 Appendix 1	Laboratory Procedure for Bacterial Test Agar

Top of Page

Part 3: Guidelines for maintaining deposits - Procedures -

Reference nb.	Procedure Title
PC/1998/3/1	Quality Control, Maintenance, Preservation, Storage and Acceptance of Cell Lines - Living Cultures -
PC/1998/3/1.1	Evaluation of Growth Colour and Morphological Characters of Callus Cultures
PC/1998/3/1.2	Standard Extraction Procedure for Callus and Suspension Cultures
PC/1998/3/1.3	Standard HPLC Procedure for Culture Extracts
PC/1998/3/1.4	Measurement of UV Spectra of unpurified Cell Extracts
PC/1998/3/1.5	Preparation HPLC Solvents for Standard Analysis
PC/1998/3/1.6	Description of Methods for Standard HPLC Analysis
PC/1998/3/2	Quality Control, Maintenance, Preservation, Storage and Acceptance of Cell Lines - Frozen Cultures -
PC/1998/3/3	Routine Maintenance and Storage of Cell Lines - Living Cell Lines -
PC/1998/3/3.1	Criteria for the Culture Transfer to Fresh Medium out of the Routine Time Schedule
PC/1998/3/3.2	Routine Procedure for the Transfer of Callus Cultures
PC/1998/3/3.3	Routine Procedure for the Transfer of Suspension Cultures to Fresh Medium
PC/1998/3/4	Routine Maintenance and Storage of Cell Lines - Frozen Cell Lines
PC/1998/3/4.1	Description and Use of the Computer Data Base for Cryocontainer Management
PC/1998/3/4.2	Description and Use of the Cryocontainer Document File (Book)

Part 3: Guidelines for maintaining deposits - Appendixes -

Reference Nb.	Appendixes
PC/1998/3/1 Appendix 1	Standard Letter: Cell Line Authentication under Suboptimum Growth Conditions
PC/1998/3/1 Appendix 2	Standard Letter: Rejection of Cell Line
PC/1998/3/1.2 Appendix 1	Measurement of Dry Weight Percentage of Fresh Cells
PC/1998/3/2 Appendix 1	Standard Letter: Unsuccessful Cryopreservation Procedure
PC/1998/3/2 Appendix 2	Standard Letter: Authentication Check for Thawed and Regrown Cultures

Top of Page

Part 4: Guidelines for delivering material to customers - Procedures -

Reference Nb.	Procedure Title
PC/1998/4/1	Delivery of cell lines

Part 4: Guidelines for delivering material to customers - Appendixes -

Reference Nb.	Appendixes
PC/1998/4/1 Appendix 1	Standard Letter: Delivery not possible because of Restrictions
PC/1998/4/1 Appendix 2	Standard Letter: Request of Import Permit
PC/1998/4/1 Appendix 3	Standard Letter: Announcement of Cell Culture Delivery

Top of Page

Guidelines prepared for CABRI by DSMZ, 20 Jan. 1998; updated August 1999
Page Layout by CERDIC
Copyright CABRI, 1998

© The CABRI Consortium 1999 - 2023
This work cannot be reproduced in whole or in part without the express written permission of the CABRI consortium.
Site maintained by Paolo Romano. Last revised on February 2023.