Guidelines
CELL LINES
PLANT CELL LINES
PLANT VIRUSES
PLASMIDS
PHAGES
MICROORGANISMS
GENOMIC LIBRARIES
PROCEDURES
CATALOGUE
LABORATORY PROCEDURES FOR MICROORGANISMS
Flow diagram
M/1998/3.00 Appendix 5.07.1
CRYOPRESERVATION OF FUNGI
Select procedure
¯
Submerge inocula in cryoprotectant in cryovial
¯
Label cryovial
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Cool with selected cooling rate (general 1°C/min.) to 45° C
store below -130 °C
¯
Check viability and quality after 16 h and 5 year (specialist)
(Revival 5 min. waterbath 30°C; Oomycota and sensitive organisms at 25° C)
Guidelines prepared for CABRI by DSMZ, CBS and BCCM, 17 May 1998
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