Guidelines
CELL LINES
PLANT CELL LINES
PLANT VIRUSES
PLASMIDS
PHAGES
MICROORGANISMS
GENOMIC LIBRARIES
PROCEDURES
CATALOGUE
LABORATORY PROCEDURES FOR ANIMAL & HUMAN CELL LINES
REFERENCE NO: AHC/1998/3/2.3/2
TITLE: PREPARATION OF CELL BANKS FOR DISTRIBUTION STOCKS
INTRODUCTION
The purpose of this protocol is to describe the procedures to be followed when preparing cell banks for distribution.
PROCEDURE
For new lines into the Collection or if existing stocks have reached less than 10 ampoules in distribution bank, remove one vial from a fully Quality Controlled Master stock.
Resuscitate (according to
Grow cell line to produce approximately 20 to 50 ampoules at between 2 to 4 x 106 cells per ampoule at 80 to 100% viability (cell counting method
AHC/1998/3/3.1/2.1).Prepare labels with cell line name, CB number, passage number if known, and date.
Cryopreserve cells according to
Store ampoules in the appropriate location in the liquid nitrogen tanks and record locations.
Update the relevant databases, giving batch number, location, batch of source material, number of ampoules and passage number if known.
Guidelines prepared for CABRI by CERDIC, DSMZ, ECACC, INRC, November 1998
Page layout by CERDIC
Copyright CABRI, 1998
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2023
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