SUBCULTURING and QUALITY CONTROL

LABORATORY PROCEDURES FOR PLASMIDS

REFERENCE NO: PP/1998/02/02

TITLE: SUBCULTURING and QUALITY CONTROL

INTRODUCTION

This document describes the basic procedure to be followed upon receipt of new plasmids for the public collection.

PROCEDURE

The deposited plasmid is subcultured and submitted to quality control tests as described in PP/1998/03/02 and PP/1998/03/03.

If all quality control tests yield satifactory results, the procedure is continued as described in PP/1998/02/03.

If there is a failure of one of the quality control tests, the depositor is informed by letter or fax and a new deposit of the plasmid is requested

If the depositor is unable to provide the collection with a new deposit of the plasmid, the accession process ends. The deposited plasmid material is returned to the depositor or with his approval removed by the collection. The recorded minimal information on the plasmid (cfr. PP/1998/02/01) is labeled with the indication that the accessioning procedure has halted. The accession number of the plasmid MAY NOT be used for another deposit.

Guidelines prepared for CABRI by BCCM/LMBP in cooperation with DSMZ and NCCB, 7 May 1998
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