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LABORATORY PROCEDURES FOR ANIMAL & HUMAN CELL LINES

Quality controls at INRC

 

Identification of cell line		Mycoplasma testing
Reference Number	Procedure Title	Reference Number	Procedure Title
AHC/1998/3/3.1/2.1	Cell enumeration by vital dye exclusion	AHC/1998/3/3.2/2.1	Mycoplasma detection by Hoescht stain
AHC/1998/3/3.1/2.2	Isoenzyme analysis	AHC/1998/3/3.2/3.1	Mycoplasma detection by nested double step PCR
AHC/1998/3/3.1/3.1	Species confirmation by direct analysis of restriction digests of genomic DNA	AHC/1998/3/3.2/3.2	Mycoplasma detection by the GibcoBRL Mycotect
AHC/1998/3/3.1/3.2	Species confirmation for human cell lines by HLA DQ Alpha amplification	AHC/1998/3/3.2/1.5	Mycoplasma eradication

 

Bacteria and fungi testing
Reference Number	Procedure Title
AHC/1998/3/3.3/2.1	Bacteria and Fungi detection by culture
AHC/1998/3/3.3/2.2	Bacteria and Fungi eradication

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