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LABORATORY PROCEDURES FOR ANIMAL & HUMAN CELL LINES
Appendix
REFERENCE NO: AHC/1998/3/1.2 Appendix 4
TITLE: INACTIVATION OF FOETAL BOVINE SERUM
INTRODUCTION
Inactivated foetal bovine serum, FBS, is used for making up media.
PROCEDURE
- Take bottle of FBS and warm to 37 ± 1°C.
- Heat water bath to 56 ± 1°C.
- Place warmed bottle of FBS in water bath at 56 ± 1°C for 30 minutes.
- Remove and allow bottle to cool.
Spray bottle with 10% alcohol before taking to tissue culture cabinet.
Guidelines prepared for CABRI by CERDIC, DSMZ, ECACC, INRC, November 1998
Page layout by CERDIC
Copyright CABRI, 1998
© The CABRI Consortium 1999 -
2023
This work cannot be reproduced in whole or in part without the express written permission of the CABRI consortium.
Site maintained by Paolo Romano. Last revised on February 2023.
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