CELL CULTURE MEDIA PREPARATION FORM

LABORATORY PROCEDURES FOR ANIMAL & HUMAN CELL LINES

Appendix

REFERENCE NO: AHC/1998/3/1.2 Appendix 2

TITLE: CELL CULTURE MEDIA PREPARATION FORM - ECACC -

Name:		Date:
Extension No:		Date Required: (min of 1 week after date requested)
Media Required:		Frequency:
Quantity:

Additional Reagents

FBS	Inactivated:	YES/NO
	Concentration:		No of Bottles:

Supplements (tick if required)

NEAA o Pyruvate o

Vitamins o Mercaptoethanol o

Hepes o HT o

HAT o Pen/Strep o

Aminopterin o Kanamycin o

Gentamycin o Neomycin o

Nystatin o

Others (give details below)

Supplement	Concentration

NB: Media is supplied in 500ml bottles - and is normally made up in batches of 10. Please try and request your media in multiples of these quantities.

(To be completed by the operator preparing the medium)

Collection Batch No:
Type of Medium:		Manufacturer:
Batch No:		Expiry Date:
No. of bottles rejected:		and reason:
No. of bottles prepared for use:
Type(s) serum:		Manufacturer(s):
Batch No(s):	(FBS)	Expiry date(s):
No of bottles used:	(FBS)
Volume of serum/500ml medium:	(FBS)
L-Glutamine batch No:		Manufacturer:
Expiry date:

Volume of Glutamine/500ml medium:
Non-essential amino acids batch no:
Manufacturer:
Expiry date:
Volume of NEAA/500ml medium:
Other additions made:
Details (i.e. batch No., Manufacturer etc.):

QC OF PREPARED MEDIUM

Batch number of TSB: ......................................................................... No of TSB Broths used ......................... incubated at 37 ± 1°C ..........R.T.........

Batch number of Thioglycollate broths: ....................................................

No of Thioglycollate broths used ............. incubated at 37 ± 1°C ...........R.T.........

Operator .......................................... Date ...........................

QC Results after ............ days incubation

All QC'd OK ...... (tick) or:

Individual bottle QC checks (if taken before end of normal QC times)
Bottle No:	Date:	QC (initial)

QC No(s) suspect/contaminated................................................................................................................

Indicate incubation temperatures in brackets after bottle No(s) or

Whole batch contaminated ........... (tick) and reject

Medium passed for laboratory use by:.......................................... Date ........................

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