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Last revised:
January 2020
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NCCB Phabagen Plasmids
Example of an entry from the NCCB_PLASMID catalogue:
| Collection_number |
NCCB 3490 |
| Name |
pZ150 |
| Other_culture_collection_numbers |
PC-V3490 |
| Type |
plasmid |
| Class |
Recombinant |
| Constructed_from |
pBR322 |
| Literature |
Zagursky, R.J. and Berman, M.L., Gene (1984) 27: 183-191 |
| History_of_deposit |
NCCB <- Hu, J.C. |
| Restricted_distribution |
No |
| Host_for_distribution |
Escherichia coli K12 JH372 |
| Medium |
Medium 171
|
| Selectable_phenotype |
Ampicillin resistance (100);Tetracycline resistance (Tc) (10) |
| Replicon |
pMB1;M13 |
| Transfer_ability |
Non-transferable |
| Host_range |
Escherichia coli vector |
| Properties_and_applications |
general cloning vector;sequencing vector;preparation of single-stranded probes |
| Copy_number |
high |
| Molecular_weight |
4.858 kb |
| Cloned_gene |
M13 Intergenic Region |
| Start_codon |
No |
| Further_information |
pZ150 contains the M13 IG fragment; the M13 intergenic region is thought to contain a cis-acting morphogenic signal for packaging and orientation of the DNA within the phage particles |
| Price_code |
1 |
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Site maintained by Paolo Romano.
Last revised in January 2020.
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